| Biological Information | |
|---|---|
| Background Information: | The PathHunter® IL-15 Bioassay Kit (Reporter) is a functional, robust, highly sensitive, and easy-to-use-cell based assay to determine the relative potency of IL-15 therapeutics. In the PathHunter IL-15 Bioassay Kit (Reporter), binding of IL-15 to the IL-15 receptor drives the expression of a reporter protein tagged with the small enhanced ProLabel® (ePL) β-galactosidase (β-gal) enzyme donor fragment. Reporter activity is measured by lysing the IL-15 Bioassay (Reporter) cells with a detection reagent containing the complementary β-gal enzyme acceptor (EA) fragment and luminescent enzyme substrate. Each PathHunter IL-15 bioassay kit contains all the materials needed to perform a complete assay, including cryopreserved, single-use cells, detection reagents, cell plating reagent,and assay plates. Note that control agonist for stimulating the cells, recombinant human IL-15, may not be included in the kit, and may be purchased separately. This bioassay has been optimized for a 96-well plate format. |
| Target Class: | Cytokine Receptor |
| Family: | IL-15 Receptor |
| Sub Family: | IL-15 |
| Accession Number: | NM_000878.3 |
| Target Name: | IL-15 |
| Target Aliases: | IL15, interleukin-15, IL-15R, IL15 Receptor, IL-15 Receptor |
| Target Species: | Human |
| Cell Background: | REH |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Signaling Reporters |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Signaling |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Inflammation/Allergy |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® IL-15 Bioassay Kit (Reporter)
The PathHunter® IL-15 Bioassay Kit (Reporter) is a functional, robust, highly sensitive, and easy-to-use-cell based assay to determine the relative potency of IL-15 therapeutics. Each PathHunter IL-15 bioassay kit contains all the materials needed to perform a complete assay, including cryopreserved, single-use cells, detection reagents, cell plating reagent, and assay plates.
User Manuals & Protocols
70-446_PathHunter IL-15 Bioassay Kit User Manual (Reporter)_ Rev0_ Final
View DocumentDatasheets
93-1178Y055_PH IL-15 Signaling Reporter Bioassay Cells_CoA_01092024
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